Mosaicism in the inner cell mass of human blastocysts.

Comprehensive analysis of karyotypic mosaicism between trophectoderm and inner cell mass

Aneuploidy has been well-documented in blastocyst embryos, but prior studies have been limited in scale and/or lack mechanistic data. We previously reported preclinical validation of microarray 24-chromosome preimplantation genetic screening in a 24-h protocol. The method diagnoses chromosome copy number, structural chromosome aberrations, parental source of aneuploidy and distinguishes certain...

Effects of Mouse Strain on Establishment of Embryonic Stem Cell Lines

Purpose: Embryonic stem (ES) cells are derived from the inner cell mass of blastocysts with self-renewal and pluripotency characteristics. These cells have potential for studies of in vitro differentiation, gene function, etc. This study was, therefore, initiated to establish new ES lines and evaluate the effects of strain on ES cell production. Materials and Methods: 3-5 day blastocysts were ...

The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells

Background A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MaterialsAndMethods In this cohort study, we used fluorescence in ...

P-86: Longer Culture of Human Blastocysts Doesn

Fate of tetraploid cells in 4n↔2n chimeric mouse blastocysts

Previous studies have shown that tetraploid (4n) cells rarely contribute to the derivatives of the epiblast lineage of mid-gestation 4n<-->2n mouse chimeras. The aim of the present study was to determine when and how 4n cells were excluded from the epiblast lineage of such chimeras. The contributions of GFP-positive cells to different tissues of 4n<-->2n chimeric blastocysts labelled with tauGF...